2024 D3922 bodipy

D3922 bodipy

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WebDec 31, 2024 · Vibratome sections were stained with DAPI to visualise nuclei and BODIPY 493/503 to visualise lipid ... D1306) and 10 mg/L BODIPY 493/503 (4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene) (Invitrogen, UK D3922) in 200 mM HEPES buffer. Slices were washed twice with 200 mM HEPES and transferred to a glass ... WebMar 29, 2024 · Further histological staining of TH+ neurons with BODIPY, a dye that specifically labels neutral lipids and is commonly used to detect LDs [30], showed that both the number and size of BODIPY+LDs in the SNpc were higher in PD mice than in controls, and were significantly decreased by Ka treatment (Fig. 3f-h).

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WebOct 27, 2024 · To monitor lipid flux, we used the fatty acid Bodipy 558/568 C12 analogue, in line with a previous protocol . In short, isolated cells were incubated for 24 h in 37°C incubator, followed by 2-h treatment with Bodipy 558/568 C12, washed 3–4 times and re-suspended in fresh DMEM (supplemented with PEST, PIA, and 3.5% BSA). WebFeb 7, 2024 · D3922: BODIPY 493: Lipid metabolism and trafficking: Neutral lipids: H34475: HCS LipidTOX Green: Lipid metabolism and trafficking: Neutral lipids: D3921: BODIPY 505: Lipid ... (BODIPY FL C5, C12 and C16) ranked among the top 15 probes, as well as all probes staining endogenous neutral lipids (BODIPY 493, ... shoe shop in hanley

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WebSep 1, 2016 · At the time-point of interest, prepare 2 μM BODIPY staining solution in PBS. The volume of staining solution required for each sample corresponds to the volume of media used for culturing cells. Wash cells with a quick rinse using 3 ml PBS to remove media/serum. Incubate on BODIPY staining solution in the dark for 15 min at 37 °C. WebBODIPY FL Dye. Invitrogen BODIPY FL dye is a bright, green-fluorescent dye with similar excitation and emission to fluorescein (FITC) or Invitrogen Alexa Fluor 488 dye. It has a … WebAug 14, 2024 · Cells were subsequently cultured with or without γ + LPS for 18 h, followed by staining with Bodipy 493/503 (#D3922, ThermoFisher, 2 μM) for 1 h, fixation in 2% … shoe shop in dawlish

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http://www.maokangbio.com/productView.action?id=10540 WebOct 21, 2024 · Once ≥90% of cells were detached, cells were washed once with PBS and stained with 5uM BODIPY 493/503 (#D3922, Invitrogen, USA) in PBS and incubated in the dark for 15 minutes at 37 °C with 5% CO 2. After incubation, cells were washed and resuspended with PBS with 5% FBS before being analysed using a BD FACSCelesta™ … shoe shop in glenferrie roadWebMar 22, 2024 · 1 Introduction Subcutaneous delivery is now generally favored as the route of administration for protein therapeutics due to improved patient access through self-administration, lower clinical costs, and the option for extended-release formulations (McLennan et al., 2005). shoe shop in culcheth warrington

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D3922 bodipy

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WebAfter 48 hours, cells were stained by BODIPY™ FL C12 (4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Dodecanoic Acid) (Thermo Fisher Scientific #D3922) to determine the amount of lipid droplet in regular conditions. We added 200µM Oleic Acid conjugated with BSA into cells and incubated for overnight. WebCatalog Number D3922 Product Name 4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene (BODIPY® 493/503) CAS Number / Name 121207-31-6 / Boron, [2-[1-(3,5-dimethyl-2H-pyrrol-2-ylidene)ethyl]-3,5-dimethyl-1H-pyrrolato-N1,N2]difluoro-, (T-4)-Molecular Formula C14H17BF2N2 Molecular Weight 262.11 Appearance red solid Lot …

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Web产品描述. BODIPY 493/503是一种亲脂性荧光探针，定位在极性脂上，能用于标记细胞中性脂内容物，特别定位在活细胞和固定细胞的脂滴上。. BODIPY 493/503与落射荧光显微镜、共聚焦荧光显微镜和双质子荧光显微镜，以及流式细胞仪兼容。. 最大激发和发射波长分别是 ... WebD3922 Print Invitrogen™ BODIPY™ 493/503 (4,4-Difluoro-1,3,5,7,8-Pentamethyl-4-Bora-3a,4a-Diaza-s-Indacene) Nonpolar structure. Supplier: Invitrogen™ D3922 ... BODIPY™ …

WebBODIPY™ 581/591 undecanoic acid can be used to detect reactive oxygen species (ROS) in cells and membranes. Oxidation of the polyunsaturated butadienyl portion of the dye … WebAug 23, 2012 · Fluorophores such as BODIPY-labeled arachidonic acid or BODIPY-conjugated electrophiles can be paired with organelle-specific probes to identify specific biological processes and signaling pathways activated in ... D3922: BODIPY FL *-503: 510-Invitrogen: D2390: BODIPY-FL-IA: Free thiols: 507: 510-Invitrogen: D6003: BODIPY …

WebJul 1, 2007 · Protocols for the detection of lipid droplets with nile red and BODIPY 493/503 are included. ... Molecular Probes #D3922) Dissolve in 5 ml ethanol . Aliquot and store at … WebApr 13, 2024 · Cells were incubated with PBS buffer containing 0.01 mg/mL DAPI (Thermo Fisher, 62248) and 2 μM BODIPY (Thermo Fisher, D3922) for 5 min before mounting in VECTASHIELD Antifade Mounting Medium. To quantify the fluorescence intensity of Plin2 or BODIPY in cells, images were taken with the same confocal set-up.

WebSep 5, 2016 · In this protocol, we describe the use of the fluorescent neutral lipid dye 4,4-difluoro-1,3,5,7,8-pentamethyl-4-bora-3a,4a-diaza-s-indacene (BODIPY 493/503) to …

WebBODIPY 505/515 rapidly permeates cell membranes of live zebrafish embryos, selectively staining cytoplasmic yolk platelets. This staining provides dramatic contrast … shoe shop in harlesden norfolkWebCatalog number: D3922. With its nonpolar structure and long-wavelength absorption and fluorescence, BODIPY™ 493/503 can be used as a stain for neutral lipds and as a tracer … shoe shop in cockermouth cumbriaWeb(D3922) BODIPY™ 493/503 (4,4-Difluoro-1,3,5,7,8-Pentamethyl-4-Bora-3a,4a-Diaza-s-Indacene) - Invitrogen Antibodies - CiteAb shoe shop in hurstpierpointWebBodipy Tm 493 503 D3922, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, … shoe shop in hungerfordWebPodocyte granularity was examined using flow cytometry, electron microscopy imaging, and BODIPY staining. Perilipin-2 protein expression was increased in podocytes exposed to presumed CPF-containing plasmas, and correlated with the capacity of plasma to induce podocyte granularity, ... (BODIPY 493/503, D3922, Thermo Fisher Scientific) ... shoe shop in gtaWebD3922: 10 mg: Price: 361,00. Your ... BODIPY™ 493/503 can be used as a stain for neutral lipds and as a tracer for oil and other nonpolar lipids. We recommend dissolving in high … shoe shop in mitchamWebJul 27, 2024 · Appropriate secondary antibodies were purchased from Invitrogen. BODIPY (493/503) was used to fluorescently label lipid droplets (2 μM, cat. #D3922, Invitrogen). 7-Aminoactinomycin D (7AAD, 0.5 μg/ml, Thermo Fisher Scientific) was used to assess cellular viability. Sulfo-N-succinimidyl oleate (SSO ... shoe shop in hatfield